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KMID : 0377519940190040399
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Chung-Ang Journal of Medicine
1994 Volume.19 No. 4 p.399 ~ p.412
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The Neuroprotective Effects of Phenytoin and Transdermal Nitroglycerin on Ischemic-Hypoxic Injury in Developing Rat Brains
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Abstract
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The goal of this study was to evaluate the neuroprotective effects of phenytoin(PH) and nitroglycerin (NTG) using in the developing rat brains. Seven days old Sprague Dawley rats underwent right carotid ligation under the halothane anesthesia and
then
they stayed in the oxygen chamber(8%) oxygen) for 3 hours in a awakened state with one of four paradigms : the control group with no medication(58%), the PH-treated group receiving intraperitoneal injection of diphenylhydantoin(30mg/kg) before
the
oxygen chamber session (17), the NTG-treated group with skin attachment of NTG(in escalating doses to 4mg/kg/hr) for 36hrs before and 48hrs post ischemia(33), and the PH plus NTG treated group(31). Temperature was controlled accurately before,
and
during hypoxia. The animals were sacrificed one week after ischemic-hypoxic injury. The weight disparties of the right and left were measured for the comparison(((L-R)/L×100). The histologic study(HE stain) was made in each animal for comparison
in
neuronal loss among the groups.
PH was effective in reducing neuronal damage in terms of weight comparison(24±2.4% damage in the control vs. 5±2.95 damage in the PH group, p<0.001) and degree of the histological changes especially in the hippocampal region(p<0.05 in CA1, CA2,
and
CA3 region). Transdermal NTG did not show any beneficial effects compared with the control group(23±3.0% vs. 24±2.5%). The PH/NTG treated group had no additional effects compared with the PH-treated group on both weight comparison(4±1.8% vs.
5±2.9%)
and histological changes. These data suggest that the degree of the injury from hypoxic-ischemic insults of developing rat brain may be reduced by the compounds that modulate voltage-dependent sodium channels such as PH but not by NTG.
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KEYWORD
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